Peronospora tabacina causes blue mold of tobacco. The pathogen causes chlorotic lesions on the tobacco leaf and bluish-gray sporangiospores can be observed on the underside of a tobacco leaf. A systemic disease in plants can also occur. Brown necrosis of the stems and stunting can occur. The ultimate goals of this research are to improve the detection and diagnosis of tobacco blue mold by developing new strategies to anticipate, prevent, and respond to the threat of disease. The specific objectives of the project are to: (1) collect and preserve blue mold isolates from epidemics in NC and the US; (2) use PCR technology for the identification of the tobacco blue mold pathogen Peronospora tabacina and other pathogens that infect tobacco; (3) examine the population structure and track migrations of the pathogen; (4) evaluate the resistance of tobacco cultivars to the disease; and (5) evaluate the use of SSR markers for genotyping isolates.
Bluemold APSNet feature
Ristaino, J. 2008. Genetic Diversity among isolates of Peronospora tabacina from tobacco. North Carolina Tobacco Research Commission, $21,761.
Ristaino, J. B. 2009. Philip Morris Undergraduate Internship for Kari Peeden, Phillip Morris Company, $6,000.
Ristaino, J. B. 2010;Population Biology of the Tobacco Blue Mold Pathogen, Peronospora tabacina. Tobacco Commission, $11,717.
Ristaino, J. B. 2011. Screening Chinese germplasm for tobacco blue mold resistance. Yunnan Tobacco Research Institute. $20,000.
Ristaino, J. B. andd Dewey, R. 2012. Development of resistance to tobacco blue mold. British American Tobacco. $20,000.
Blanco, M., Ivors, K., Carbone, I. and Ristaino, J. 2014. Tracking regional migrations of the tobacco blue mold pathogen, Peronospora tabacina in North America, Central America, the Carribean and Europe using gene genealogies. Mol. Ecol. In preparation
Trigiano, R. N., Wadl, P. A., Dean, D., Johnson, D., Scheffler, B. E., Runge,F., Telle, S., Thines, M., Ristaino, J, and Spring, O. 2012. Twelve Polymorphic Microsatellite Loci for Peronospora tabacina, the Cause of Tobacco Blue Mold. Mycologia 104:633-640.
Blanco, M and Ristaino, J. B. 2011. A real-time PCR method for detection of Peronospora tabacina in tobacco. ;Plant Dis. 95: 673 682.
Ristaino, J. B., Johnson, M., Blanco-Meneses, M., Lui, B. and 2007. Identification of the Tobacco blue mold pathogen, Peronospora tabacina by PCR. Plant Dis. 91:685-691.