Ristaino Lab Protocols
Isolation and Maintenance of Phytophthora species
- Isolation of Phytophthora species
- Media Recipes
- Live leaf maintenance of P. infestans
- Phytophthora storage methods
DNA Extraction
- DNA extraction with Qiagen DNEasy kit
- Modified CTAB extraction for herbarium specimens
- FTA card extraction
- NaOH extraction
- Quick extraction using microneedle patches
PCR Protocols
- Identification of P. infestans using ITS
- Identification of P. infestans using COXI
- Identification of Phytophthora species using ITS
- Mitochondrial haplotyping by sequencing of P. infestans
- Electrophoresis gel recipes
- Buffer recipes
- PCR product clean up with ExoSAP-IT
Microsatellites
Miscellaneous
- Cryogenic backup and recovery of bacteria
- Long term storage for fungal cultures
- Storing Mycosphaerella in liquid nitrogen